Reporter

Part:BBa_J10068:Design

Designed by: Malcolm Campbell   Group: iGEM2005   (2012-01-10)


luciferase + LRE + BD18 original RBS removed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2621
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 2057
    Illegal SpeI site found at 2622
    Illegal PstI site found at 2636
    Illegal NotI site found at 2629
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2622
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2622
    Illegal PstI site found at 2636
    Illegal AgeI site found at 897
    Illegal AgeI site found at 2599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal SapI.rc site found at 329
    Illegal SapI.rc site found at 2113


Design Notes

removed RBS to allow C-dog system to work.


Source

from registry parts, synthesized de novo

References